Regulatory

Part:BBa_J100250

Designed by: Jeffrey Burton   Group: Campbell_M_Lab   (2016-01-28)


trp promotor lab

We are inserting the TRP promoter into E. coli and monitoring the natural production of tryptophan. TRP is a repressible promoter which causes a negative feedback mechanism--the continued production of tryptophan with inhibit the TRP promoter which will then cut off the further production of tryptophan. We will observe the maximum threshold of the E. coli bacteria before the tryptophan levels increase enough to inhibit the TRP promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None