Coding

Part:BBa_J100239

Designed by: Nicholas Elder   Group: Campbell_M_Lab   (2015-11-09)


tCloneTetRed with scrambled riboswitch

This is based on the tConeTetRed insert (part BBa_J119386). The insert had the P2 promoter and GFP gene removed by digestion with BsaI and has had a scrambled sequence of a known riboswitch ligated in its place. This was used to test the effectiveness of tConeTetRed without the presence of a riboswitch, but with the added genetic distance of a functional riboswitch between the promoter and RBS. This was tested in JM109 E. coli cells and produces measurable fluorescence when grown in the presence of Tetracycline (20 ug/mL).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 355
    Illegal SpeI site found at 100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 501
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 100
    Illegal NgoMIV site found at 527
    Illegal NgoMIV site found at 895
    Illegal NgoMIV site found at 1055
    Illegal AgeI site found at 2002
    Illegal AgeI site found at 2114
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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