Device
Part:BBa_J100224
Designed by: Julia Preziosi Group: Campbell M Lab (2015-07-07)
Theophylline Aptamer from PDB with RS3, riboswitch in tCloneRed
This part comes from the combination of a theophylline aptamer, as determined by the Protein Data Bank, with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, theophylline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 756
Illegal AgeI site found at 868 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |