Device

Part:BBa_J100222

Designed by: Julia Preziosi   Group: Campbell M Lab   (2015-07-07)


Xanthine Aptamer from riboCalc with RS3, riboswitch in tCloneRed

This part comes from the combination of a xanthine aptamer, as determined by the Riboswitch Calculator, with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, xanthine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 755
    Illegal AgeI site found at 867
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None