Device

Part:BBa_J100219

Designed by: Julia Preziosi   Group: Campbell_M_Lab   (2015-06-24)


tCloneTet with Riboswitch 8

Using Golden Gate Assembly, the transcriptional riboswitch RS8 (from Wachsmuth, et al.) was ligated into the destination vector tClone Tet (J119374) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine. Based on the previous research of Wachsmuth, et al., we do not expect this to be a very effective riboswitch (small dynamic range). We will be using it as a negative control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 891
    Illegal NgoMIV site found at 1051
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None