Device

Part:BBa_J100215

Designed by: Julia Preziosi   Group: Campbell M Lab   (2015-06-24)


tCloneTet+Red fusion protein reporter with Riboswitch 4

This is a hybrid taken from part J119386, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, the fusion protein Tet+RFP) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 528
    Illegal NgoMIV site found at 896
    Illegal NgoMIV site found at 1056
    Illegal AgeI site found at 2003
    Illegal AgeI site found at 2115
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None