Device

Part:BBa_J100214

Designed by: Julia Preziosi   Group: Campbell M Lab   (2015-06-22)


tCloneTet+Red fusion protein reporter with Riboswitch 10Shift

This is a hybrid taken from part J119386, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, the fusion protein Tet+RFP) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 538
    Illegal NgoMIV site found at 906
    Illegal NgoMIV site found at 1066
    Illegal AgeI site found at 2013
    Illegal AgeI site found at 2125
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None