Device

Part:BBa_J100213

Designed by: Julia Preziosi   Group: Campbell M Lab   (2015-06-22)


tCloneTet+Red fusion protein reporter with Riboswitch 3Shift

This is a hybrid taken from part J119386, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, the fusion protein Tet+RFP) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 551
    Illegal NgoMIV site found at 919
    Illegal NgoMIV site found at 1079
    Illegal AgeI site found at 2026
    Illegal AgeI site found at 2138
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None