Device
Part:BBa_J100209
Designed by: Shuk Hang Li Group: Campbell M Lab (2015-06-02)
tClone Red RS8
This sequence has Isolated pUC-IDT from tClone Red (J119361) by removing sequence in between the BsaI restriction sites and then RS8 riboswtich (from Wachsmuth et al.) was ligated. A ligand such as theophylline can bind to the sequence and allow transcription to take place. This is not expected to be a highly functional riboswitch. It is used as negative control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 756
Illegal AgeI site found at 868 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |