Device

Part:BBa_J100209

Designed by: Shuk Hang Li   Group: Campbell M Lab   (2015-06-02)

tClone Red RS8

This sequence has Isolated pUC-IDT from tClone Red (J119361) by removing sequence in between the BsaI restriction sites and then RS8 riboswtich (from Wachsmuth et al.) was ligated. A ligand such as theophylline can bind to the sequence and allow transcription to take place. This is not expected to be a highly functional riboswitch. It is used as negative control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 756
    Illegal AgeI site found at 868
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None