Device

Part:BBa_J100207

Designed by: Camille Johnson   Group: Campbell M Lab   (2015-06-02)


tClone Red RS3

We took out everything between the BsaI sites in J119361. We then inserted the RS3 riboswitch, the sequence of which was found in De novo design of a synthetic riboswitch that regulates transcription termination (Wachsmuth et. al).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 765
    Illegal AgeI site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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