Regulatory

Part:BBa_J100200

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2014-10-13)


tClone tetA v2

This device is used to test new transcriptional termination riboswitches. The default color of colonies would be green and they would be tetracycline sensitive. Users employ golden gate assembly (GGA) to remove the cassette flanked by BsaI sites and clone into this location their transcriptional termination riboswitch. In the presence or absence of ligand, the contract would become tetracycline resistant, or not, depending if the riboswitch was an on or off switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1310
    Illegal NgoMIV site found at 1678
    Illegal NgoMIV site found at 1838
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 899
    Illegal BsaI.rc site found at 42


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Categories
Parameters
None