Measurement

Part:BBa_J100199

Designed by: Telavive Taye   Group: Campbell_M_Lab   (2014-09-15)


Fitness Gene Tester + LVA degradation tag

We designed a fitness gene tester that allows for easy ligation of a fitness gene downstream of a theophylline riboswitch. Easy ligation is facilitated by the BsaI sites separated by a spacer sequence. The chosen fitness gene inserted into the fitness gene tester will include an LVA degradation tag that will reduce the half life of the fitness gene being tested.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
    Illegal AgeI site found at 107
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197
    Illegal BsaI.rc site found at 171


[edit]
Categories
Parameters
n/aFitness Gene Tester + LVA degradation tag