Part:BBa_J100181

soxS promoter

Under superoxide stress, the SoxR protein is activated. This triggers the transcription of a second regulatory gene, soxS, when its base sequence is fully intact. The function of this process is to activate superoxide stress genes in Escherichia coli. When soxS is artificially shortened, however, the binding of the SoxR protein turns the promoter off, making it repressible. In this experiment, the absence of the SoxR protein under chemical conditions should allow the shortened soxS promoter to remain active.

Hidalgo & Demple. "Spacing of promoter elements regulates the basal expression of the soxS gene and converts SoxR from a transcriptional activator into a repressor." The EMBO Journal. Vol. 16 No. 5 pp. 1056-1065. (1997)

Shin, Singh, Cheon, & Roe. "Activation of the SoxR Regulon in Streptomyces coelicolor by the Extracellular Form of the Pigmented Antibiotic Actinorhodin." Journal of Bacteriology. p. 75-81 (Jan. 2011)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]