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aspA promoter

Promoter for the aspA-dcuA operon. The aspA gene codes for the enzyme aspartoacylase, which is essential to the production of white matter in the brain. This enzyme breaks down N-acetyl-L-Aspartic Acid into separate aspartic acid and acetic acid. The promoter is repressible when nitrate is added and the protein NarL can bind at the repressor sites -60 and -115. Because we cut down the length of the promoter to only 54 base pairs in length, the protein binding sites to repress the promoter have been removed. NarL will be unable to bind and repress the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None