DNA

Part:BBa_J100174

Designed by: Hannah Itell   Group: Campbell M Lab   (2014-07-25)


Strong Promoter + M6-R1 Ammeline Riboswitch + RFP

This has a seven mutations from the original M6 riboswitch sequence found in part J119332. Five of these mutations are part of the intentional design of the riboswitch, in order for it to be more effective than the original M6 riboswitch. As with part J119332, base 432 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS. The two additional mutations are T20C in the promoter region and C852T in the RFP CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
    Illegal AgeI site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None