DNA

Part:BBa_J100171

Designed by: Jonathan Lim   Group: Campbell M Lab   (2014-07-10)

Strong Promoter + M6"-R3 Ammeline Riboswitch + RFP

This construct is a basic biosensor for ammeline, based on part J119335. The P5 promoter drives the M6"-R3 riboswitch, which permits translation of the reporter gene (RFP) in the presence of ammeline.

The M6"-R3 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).

Mutation at position 432 nt of A to G changes tyrosine (Y) to cysteine (C) in RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
    Illegal AgeI site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None