DNA

Part:BBa_J100166

Designed by: Kathryn Smith   Group: Campbell M Lab   (2014-07-02)


P5 promoter + M6''-R10 Ammeline Riboswitch + RFP

This riboswitch sequence was built off of the original riboswitch sequence found in part J119335. It was designed to be a more effective riboswitch. The sequence contains three mutations from the original part. Two of which were designed (C to G at 77 and a C insert at 183) and one which occurred accidentally (T to C at 564). The accidental mutation caused no amino acid change (E. coli codon bias change from .34 to .4).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None