Reporter

Part:BBa_J100165

Designed by: Dustin Atchley   Group: Campbell M Lab   (2014-07-02)


High promoter+High C dog+dnaG gene

This part is a strong P5 promoter and a strong BD24 C dog followed by E. coli dnaG. The P5 and BD24 are from a previously assembled part S05028.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1892
    Illegal suffix found in sequence at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1892
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NotI site found at 12
    Illegal NotI site found at 1898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1892
    Illegal XhoI site found at 383
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1892
    Illegal suffix found in sequence at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1892
    Illegal XbaI site found at 1907
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 404


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