DNA

Part:BBa_J100163

Designed by: Hannah Itell   Group: Campbell M Lab   (2014-06-25)

Strong Promoter + M6-R10 Ammeline Riboswitch + RFP

This has a two mutations from the original riboswitch sequence found in part J119332. This version is designed to be a more effective riboswitch. As with part J119332, base 431 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aStrong Promoter+ M6-R10 Ammeline Riboswitch+ RFP