DNA

Part:BBa_J100143

Designed by: Catherine Doyle   Group: Campbell M Lab   (2014-04-18)


Caffeine Riboswitch1

This part combines Caffeine Riboswitch 1 and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3144
    Illegal suffix found in sequence at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3144
    Illegal SpeI site found at 939
    Illegal PstI site found at 953
    Illegal NotI site found at 946
    Illegal NotI site found at 3150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3144
    Illegal suffix found in sequence at 939
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3144
    Illegal XbaI site found at 3159
    Illegal SpeI site found at 939
    Illegal PstI site found at 953
    Illegal AgeI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197
    Illegal SapI.rc site found at 230


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