Generator

Part:BBa_J100135

Designed by: Elizabeth Brunner   Group: Campbell M Lab   (2013-10-07)


thyA Gene

This construct includes promoter P5 + C-Dog BD24 (bi-cistronic RBS) + thyA gene (BBa_J100132). This gene is used to complement the mutation in E. coli strain C600thyA-::Kan^R (Thompson et al., 2002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 734
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None