Coding

Part:BBa_J100132

Designed by: Malcolm Campbell   Group: Campbell_M_Lab   (2013-09-23)


E. coli thyA

This is the E. coli thyA gene that converts dUMP to dTMP. Cells lacking this gene (C600thyA::KanR) need this cell to be prototrophic.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 841
    Illegal PstI site found at 855
    Illegal NotI site found at 7
    Illegal NotI site found at 848
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 841
    Illegal PstI site found at 855
    Illegal AgeI site found at 637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 834


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Categories
Parameters
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