Coding

Part:BBa_J100131

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-09-23)


human IL-6

Human IL-6 CDS optimized for E. coli with no internal restriction sites for EcoRI, XbaI, SpeI, PstI or BsaI. This insert was synthesized by GeneArt and was cloned into their pMK-T plasmid so we had the BioBrick prefix and suffix included in the basic part sequence. It will be used with Golden Gate Assembly to put downstream of promoter+RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 604
    Illegal PstI site found at 618
    Illegal NotI site found at 7
    Illegal NotI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 604
    Illegal PstI site found at 618
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 597


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