DNA

Part:BBa_J100121

Designed by: Phoebe Parrish, Meredith Simpson, A. Malcolm Campbell   Group: Campbell M Lab   (2013-07-31)


Promoter + Spinach + BsaI sites

This part contains a promoter, Spinach, and two BsaI sites with a spacer in between them. Spinach is a fluorogen-activated RNA sequence (biosensor) that binds to DFHBI and fluoresces green when a downstream mRNA sequence is produced. This part can be used with Golden Gate Assembly to determine whether a gene is being transcribed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 553
    Illegal PstI site found at 256
    Illegal NotI site found at 205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal PstI site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 246
    Illegal BsaI site found at 266
    Illegal BsaI.rc site found at 20


[edit]
Categories
Parameters
None