Composite

Part:BBa_J100120

Designed by: jessica gronniger   Group: Campbell M Lab   (2013-07-29)


J100116 with P5 Promoter Deleted

AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). C. dog BD24 (J119028) was ligated between junctions B and C on scaffold. P5 promoter between junctions A and B was deleted. Golden Gate assembly was used to put the C. dog and adhE into the scaffold. iPCR was used to generate the receiving plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2808
    Illegal PstI site found at 2822
    Illegal NotI site found at 2815
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2808
    Illegal PstI site found at 2822
    Illegal AgeI site found at 707
    Illegal AgeI site found at 1931
    Illegal AgeI site found at 1976
  • 1000
    COMPATIBLE WITH RFC[1000]


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