Coding

Part:BBa_J100116

Designed by: jessica gronniger   Group: Campbell M Lab   (2013-07-17)


AdhE gene cloned into J100112

AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put adhE into the gap between junctions C and D. iPCR was used to generate the receiving plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2843
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2844
    Illegal PstI site found at 2858
    Illegal NotI site found at 2851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2844
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2844
    Illegal PstI site found at 2858
    Illegal AgeI site found at 743
    Illegal AgeI site found at 1967
    Illegal AgeI site found at 2012
  • 1000
    COMPATIBLE WITH RFC[1000]


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