Coding
Part:BBa_J100116
Designed by: jessica gronniger Group: Campbell M Lab (2013-07-17)
AdhE gene cloned into J100112
AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put adhE into the gap between junctions C and D. iPCR was used to generate the receiving plasmid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 2843
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2844
Illegal PstI site found at 2858
Illegal NotI site found at 2851 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2844
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2844
Illegal PstI site found at 2858
Illegal AgeI site found at 743
Illegal AgeI site found at 1967
Illegal AgeI site found at 2012 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |