Coding

Part:BBa_J100113

Designed by: jessica gronniger   Group: Campbell M Lab   (2013-07-03)


sfGFP cloned into J100112

sfGFP (J100070) ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put the sfGFP into the gap between junctions C and D. iPCR was used to generate the receiving plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
  • 1000
    COMPATIBLE WITH RFC[1000]


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