Generator

Part:BBa_J100108

Designed by: Erin McGuire   Group: Campbell M Lab   (2013-03-12)


Riboswitch and GFP +High production of eCDM8

High promotor and low RBS (c-Dog). E. coli optimized caffeine demethylase with the same amino acid sequence as yCDM8 from Michener and Smolke (2012). Theophylline sensitive riboswitch mediates the production of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 4162
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4163
    Illegal PstI site found at 4177
    Illegal NotI site found at 4170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 4163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4163
    Illegal PstI site found at 4177
    Illegal AgeI site found at 100
    Illegal AgeI site found at 184
    Illegal AgeI site found at 1083
    Illegal AgeI site found at 1744
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 172


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