Composite

Part:BBa_J100101

Designed by: Erin McGuire   Group: Campbell M Lab   (2012-11-13)


High production of eCDM8

High promotor and high RBS (c-Dog). E. coli optimized caffeine demethylase with the same amino acid sequence as yCDM8 from Michener and Smolke (2012).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3276
    Illegal PstI site found at 3290
    Illegal NotI site found at 3283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3276
    Illegal PstI site found at 3290
    Illegal AgeI site found at 196
    Illegal AgeI site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]


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