Regulatory

Part:BBa_J100099

Designed by: Phoebe Parrish   Group: Campbell M Lab   (2012-09-13)

A promoter (CydAB) activated by the FNR enzyme

The promoter, CydAB, was found to be activated by the FNR enzyme, which is induced by the presence of (NH4)2Fe(SO4)2 and ascorbate. The oligo includes both CydAB, the FNR binding site, and the sticky ends needed for the Golden Gate Assembly method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Jeffrey Green. 1993. "Activation of FNR-dependent transcription by iron: An in vitro switch for FNR." FEMS Microbiology Letters 113 (1993) 219-222

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Parameters
n/aA promoter (CydAB) activated by the FNR enzyme