Regulatory

Part:BBa_J100097

Designed by: Sarah Kim   Group: Campbell M Lab   (2012-09-13)

Anhydrotetracycline inducible promoter with BsaI sticky ends

This promoter sequence contains BsaI sticky ends and is anhydrotetracycline inducible. Additional information about how the sequence was isolated and its regulatory uses can be found in Rolf Lutz and Hermann Bujard's paper, "Independent and Tight Regulation of Transcriptional Units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 Regulatory Elements" in Nucleic Acids Research, 1997, Vol. 25, No. 6 1203-1210 which can be accessed at http://www.ncbi.nlm.nih.gov/pubmed/9092630.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAnhydrotetracycline inducible promoter with BsaI sticky ends