Part:BBa_J100090
CRISPR sequence with GFP and AmpR targets
The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) has been shown to act as a bacterial immune system. This system is characterized by short repeats, called CRISPR repeats, and in between these repeats are short, unique segments of DNA that have been acquired from foreign DNA that particular bacteria or archaea has encountered. E.coli, as well as other bacteria and archaea can use these "target spacer" segments to attack and destroy the foreign objects that contain that DNA, thereby functioning as an immune system. This part is an engineered synthetic CRISPR system to be used in E.coli. There is a leader sequence at the beginning of this sequence that acts as a promoter for transcription. The target spacers are short, 30 bp sequence that targets the GFP gene (part E0040) in 2 places (near the beginning and end of the gene), as well as spacers that target the Ampicillin resistance gene (part J119043) near the beginning and end of the gene.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 392 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 393
Illegal PstI site found at 407
Illegal NotI site found at 7
Illegal NotI site found at 400 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 393 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 393
Illegal PstI site found at 407 - 1000COMPATIBLE WITH RFC[1000]
| None |