Part:BBa_J100085
short CRISPR sequence with GFP target spacer
The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) has been shown to act as a bacterial immune system. This system is characterized by short repeats, called CRISPR repeats, and in between these repeats are short, unique segments of DNA that have been acquired from foreign DNA that particular bacteria or archaea has encountered. E.coli, as well as other bacteria and archaea can use these "target spacer" segments to attack and destroy the foreign objects that contain that DNA, thereby functioning as an immune system. This part is an engineered synthetic CRISPR system to be used in E.coli. The one target spacer is a short, 30 bp sequence that targets the GFP gene (part E0040). This part also includes a BamHI restriction enzyme recognition site, for use in expanding this sequence if needed.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 220
Illegal XbaI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 221
Illegal PstI site found at 235
Illegal NotI site found at 6
Illegal NotI site found at 228 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 214
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 221
Illegal XbaI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 15
Illegal SpeI site found at 221
Illegal PstI site found at 235 - 1000COMPATIBLE WITH RFC[1000]
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