Composite

Part:BBa_J100083

Designed by: Betsy Gammon   Group: Campbell M Lab   (2012-07-12)


LuxI Long + RBS + GFP

This is the long form of the pLux promoter. This promoter promotes the luxR gene but only in the presence of 30C6. The shorter form of the pLux promoter does not include an arcA binding site. Phosphorylated arcA typically represses this long promoter in anaerobic conditions. This promoter is also attached to a medium strength RBS and a GFP gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 868


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Categories
Parameters
None