Device

Part:BBa_J100079

Designed by: Rebecca Evans   Group: Campbell M Lab   (2012-07-07)

Riboswitch and GFP

This part combines Riboswitch D (Part J100065) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 172


[edit]
Categories
Parameters
n/aRiboswitch and GFP