Deleted
Sample Discontinued
Experience: None
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Measurement

Part:BBa_J100050

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2012-01-10)


pBad+BD18+RFP_withoutATG

This construct allows users to compare the effect of the pBad promtoer with two other constructs with similar design but different promoters. It contains the C-dog bicistronic piece J119024 plus RFP (E1010) but the original start codon has been deleted since BD18 provides a new start codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 787
    Illegal AgeI site found at 899
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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