Coding

Part:BBa_J100014

Designed by: Eric Sawyer   Group: Campbell M Lab   (2011-06-21)


LuxI with 2 Clause 2-SAT Problem

LuxI is frameshifted by the addition of a SAT problem between the engineered restriction sites AsiSI and AscI. The correct reading frame is restored only if suppressor tRNA CCACU (K199002) or suppressor tRNA CUAGU (K199001) AND suppressor tRNA CGGUC (K199028) or suppressor tRNA CCACU (K199002) are present in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 290
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None