Coding

Part:BBa_J100013

Designed by: Eric Sawyer   Group: Campbell_M_Lab   (2011-06-21)


LuxI with 1 Clause 2-SAT Problem

LuxI is frameshifted by the addition of a SAT problem between the engineered restriction sites AsiSI and AscI. The correct reading frame is restored only if suppressor tRNA CCACU (K199002) or suppressor tRNA CUAGU (K199001) is expressed in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 276
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None