Intermediate

Part:BBa_J100007

Designed by: Eric Sawyer   Group: Campbell_M_Lab   (2011-05-24)


pLac+RBS+LoxP+Stop Sequence+LoxP

This stop sequence blocks transcription and translation by the formation of a stem-loop structure. In the presence of Cre recombinase, the stop sequence is excised from the DNA. A protein coding part placed downstream of this part will be expressed only in the presence of Cre.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 284


[edit]
Categories
Parameters
None