Intermediate

Part:BBa_J100006

Designed by: Eric Sawyer   Group: Campbell M Lab   (2011-05-24)


LoxP+Stop Sequence+LoxP

This stop sequence forms a stem-loop in the mRNA, inhibiting transcription and translation. In the presence of Cre recombinase, the stop sequence is excised from the DNA and therefore rendered non-functional.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 56


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Categories
Parameters
None