Generator

Part:BBa_J100003

Designed by: Eric Sawyer   Group: Campbell M Lab   (2011-05-24)


pTet+RBS+Cre2SAT1Clause

Produces functional (in-frame) Cre. The Cre ORF is modified to include AsiSI and AscI sites on the 5' end after the start codon. Because of a spacer, Cre is in-frame.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 468
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None