Composite

Part:BBa_J100002

Designed by: Eric Sawyer   Group: Campbell_M_Lab   (2011-05-24)


pTet+RBS+Cre2SAT1Clause+pLpp+tRNA CGGUC

Suppressor tRNA CGGUC (K199028) is produced under regulation by the lpp promoter (K199106). The tRNA does not suppresses either of the frameshift mutations in Cre, causing Cre to be frameshifted and nonfunctional. We used this part as a control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 468
    Illegal BamHI site found at 1215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
    Illegal AgeI site found at 1252
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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