Generator

Part:BBa_J07086

Designed by: Annie Vo   Group: iGEM2005   (2005-09-28)


tetR promoter+rbs+scfv-FecA 567-576+rbs+FecI+rbs+FecR'+terminator

This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. FecA' aa 567 to 576 are deleted and replaced by scFv. All are under control of tetR promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1357
    Illegal BglII site found at 1848
    Illegal BglII site found at 3059
    Illegal XhoI site found at 3548
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI.rc site found at 3551


[edit]
Categories
//classic/generator/uncategorized
Parameters
None