Generator
Part:BBa_J07084
Designed by: Annie Vo Group: iGEM2005 (2005-09-28)
tetR promoter+rbs+scfv-FecA 176+rbs+FecI+rbs+FecR'+terminator
This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. Fusion is at aa 176 of FecA' protein. All are under control of tetR promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 678
Illegal BglII site found at 2176
Illegal BglII site found at 3089
Illegal XhoI site found at 3578 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1296
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 156
Illegal BsaI.rc site found at 3581
[edit]
Categories
Parameters
//classic/generator/uncategorized
None |