Generator

Part:BBa_J07082

Designed by: Annie Vo   Group: iGEM2005   (2005-09-28)


tetR promoter+rbs+scfv-FecA 155+rbs+FecI+rbs+FecR'+terminator

This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. Fusion is at aa 155 of FecA' protein. All are under control of tetR promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 615
    Illegal BglII site found at 2176
    Illegal BglII site found at 3089
    Illegal XhoI site found at 3578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1233
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI.rc site found at 3581


[edit]
Categories
//classic/generator/uncategorized
Parameters
None