Device

Part:BBa_J07024

Designed by: jenmitch   Group: iGEM2005   (2005-07-14)


ToxR'::scFv Fusion 1 (with promoter and rbs and terminator)

The first of three fusions between ToxR', a modified dimerizing protein, and an anti-fluorescein binding scFv sequence. ToxR', active as a dimer, binds to the ctx promoter sequence (BBa_J07007) to initiate transcription. This part is designed to respond to a fluorescein dimer and activate the ctx promoter.

The restriction sites flagged below were introduced to allow for protein fusions, and are not to be removed. See ToxR' (J07009) for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 791
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/device/uncategorized
Parameters
None