Signalling

Part:BBa_J07012

Designed by: mit_igem_2005   Group: iGEM2005   (2005-07-08)


scFv anti-fluorescein [4-4-20]

single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the weakest of the three specified sequences.

DNA insert in the pCT-4-4-20 (also known as pcT-302) from NheI to XhoI


Usage and Biology

Does not have a start codon because it was designed to be fused to the c-terminus of another protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/cellsignalling
Parameters
None