Reporter
mCherry

Part:BBa_J06505:Design

Designed by: ytwang   Group: iGEM2005   (2005-07-18)


monomeric RFP optimized for bacteria

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR.


Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.



Source

mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.

References