Reporter
mCherry
Part:BBa_J06505:Design
Designed by: ytwang Group: iGEM2005 (2005-07-18)
monomeric RFP optimized for bacteria
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR.
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
Source
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.