Coding
cI857

Part:BBa_J06503

Designed by: ytwang   Group: iGEM2005   (2005-06-28)


Lambda cI857 (Lambda II) (RBS- LVA+)

Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.


Usage and Biology

Introduced two point mutations: G(352)->A for ind- and G(199)->A for 857 temperature sensitivity. Mutations obtained from Hendrix et al. Lambda II.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
//cds
Parameters
None