Coding
lacI ts

Part:BBa_J06501

Designed by: ytwang   Group: iGEM2005   (2005-06-14)

LacI repressor (temperature-sensitive mut 265) (+LVA)

Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature-sensitive. Mutation from Chao, et al., 2002: Gly265->Asp (GG(803)T->GAT)

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligandsallolactose, IPTG
proteinLacI-mut2
swisspro~ P03023
tagLVA